Simultaneous determination of paracetamol and para-aminophenol using activated glassy carbon electrode

Abstract

The study of electrochemical determination of paracetamol and para-aminophenol was investigated by electroanalytical techniques such as CV and DPV on activated glassy carbon electrode. The GCE was activated by applying a potential of 1750 mV for 200 s in time based technique phosphate buffer solution followed by potential cycling 0.0 to 700 mV for six cycles. Activated GCE was chosen for simultaneous determination of paracetamol and para-amino phenol and it showed an excellent electrocatalytic effect and decreases the overpotential than bare GCE. Electrochemical reaction of APAP and PAP at AGCE was found to be reversible involving the transfer of two electron and two proton processes. Phosphate buffer solution pH 6 was used as a solvent and supporting electrolyte for the analysis of standard paracetamol and para-aminophenol throughout the experiment. The peak to peak separation of APAP and PAP is 271.1 mV, which is sufficient for simultaneous determination of the two analytes. The electrochemical reaction of APAP and PAP was adsorption controlled process. The oxidation peak current linearly dependent on the concentration of APAP in the range of 4 mol L-1 to 60 mol L-1 at constant concentration of PAP and concentration of PAP in range of 10 mol L-1 to 240 mol L-1 at constant concentration of APAP. The LOD of APAP and PAP were found to be 1.46 x 10-7 mol L-1 and 3.24 x 10-7mol L-1 respectively and LOQ of APAP and PAP were 4.87 x 10-7 mol L-1 and 1.08 x10-6 mol L-1 respectively. Validation of the method was also checked with recovery studies and interferences were also studied.