Square Wave Voltammetric Determination of Ibuprofen at Poly(L-Aspartic Acid) Modified Glassy Carbon Electrode

Abstract

Abstract— A simple and fast modification of bare glassy carbon electrode (GCE) by electropolymerization of L-aspartic acid (L-Asp) using cyclic voltammetry was reported for the detection of ibuprofen (IBP). The poly(L-Asp)/GCE was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and electroactive surface area measurements. The cyclic voltammetric and square wave voltammetric study of IBP in 0.25-M acetate buffer solution at pH 4 showed an obvious electrocatalytic effect toward IBP oxidation, which resulted in a higher current response and a negative shift in the peak potential, at the polymer film modified electrode compared with the bare GCE. Under the optimized conditions, a calibration curve was obtained by the square wave voltammetry at the poly(L-Asp)/GCE. The square wave voltammetric peak current linearly varied with IBP concentrations in the range of 1.0 × 10−6 M to 1.5 × 10−4 M with the limit of detection (3Sb/m) and the limit of quantification (10Sb/m) of 2.2 × 10−7 and 7.4 × 10−7 M, respectively. The method was successfully applied for the determination of IBP in pharmaceutical formulations and biological samples with satisfactory recovery values, 90%–108%. The proposed sensor also demonstrated good selectivity, repeatability, and reproducibility with appreciable long term stability. Index Terms—Poly(L-aspartic acid), ibuprofen, tablets, urine, square wave voltammetry.