Abstract:
Rhamnus prinoides (Gesho) is an angiosperm dioecious plant which is used for cash income, as a
bittering agent for the preparation of the local alcoholic beverage and medicinal values. Although
it has intense use, still its practical application is limited for local alcoholic beverage preparation,
and no sufficient scientific report on the level and quantity of bittering agents, essential oils, and
antioxidants of this plant. Therefore, the objective of this research was to assess and characterize
the bittering agents, essential oils, and antioxidants of R. prinoides. The leaves of R. prinoides
were collected from eleven different sample sites and allowed to sun dry and then powdered. It
was further dried in an oven at 600C for 1 h and then total resin, essential oils, alpha-acid, isoalpha acid, beta-acid, flavonoids, and polyphenols of R. prinoides were determined. Based on
this study finding, (16.03 ± 0.03-17.05 ± 0.04%) total resins, (1.82 ± 0.11-3.14 ± 0.02 mg/l)
alpha acids, (2.26 ± 0.15- 4.15 ± 0.10 mg/l) iso-alpha acids, (2.62 ± 0.04-4.29 ± 0.08 mg/l) beta
acids, (7.74 ± 0.11-12.47 ± 0.13%) essential oils, (23.00 ± 0.14-25.01 ± 0.09%) flavonoids, and
(2.11 ± 0.01-2.41 ± 0.02%) polyphenols were obtained in the leaves of R. prinoides depending
on sample collection sites. R. prinoides leaves taken from Tikledingay has shown statistically (P
≤ 0.05) significant amount of soft resin (11.23 ± 0.07%), alpha acid (3.14 ± 0.02 mg/l), iso-alpha
acid (4.15 ± 0.10 mg/l), essential oils (12.47 ± 0.13%), flavonoids (25.01 ± 0.09%), and
polyphenols (2.41 ± 0.02%) in comparison with other samples. Generally, R. prinoides is a good
source of bittering agents, essential oils, and antioxidants. However, the quantity of bittering
agents, essential oils, and antioxidants in the leaves of R. prinoides were varied with sample
collection sites. So, R. prinoides can be used as a substitute for commercial hops used in the
breweries and promotion should be done to introduce the potential of gesho as a bittering agent,
source of aroma and flavor, and also antioxidant for beer, and other alcoholic beverages
