PHYTOCHEMICAL INVESTIGATION AND ANTIMICROBIAL ASSAY OF LEAVE EXTRACT OF ZEHNERIA SCABRA

Abstract

The genus zehneria have been under use since long time for the treatment of various diseases such as stomach pain, fever, skin disease, diarrhea, analgesic, bacteria and antibiotic properties. Zehneria Scabra is one of the medicinal plants used traditionally for the treatment of different disease. This study intended to identify the chemical constituents of the 99% CH3OH leave extract of zehneria scabra. The phytochemical screening of methanol crude extract indicated the presence of alkaloid, flavonoid, terpenoids, glycosides, tannin, phenol and absence of carbohydrate and saponins. The crude extract fractions were tested against two bacterial species (S. Aureus and E.Coli) using agar well diffusion method at 100mg/ml concentrations in the presence of positive control. The chloroform and ethyl acetate fraction revealed more potent antibacterial activities against the growth of the bacterial strains than other solvent fraction of methanol crude extract. Isolation and purification of the crude extract was conducted by using preparative thin layer chromatography (10% ethylacetate:90% chloroform).The structure of the isolated component was determined by using a combination of spectroscopic techniques such as, IR, 1H NMR, 13CNMR and DEPT-135 Spectra.