Abstract:
Background: Pain and inflammation are the major devastating health problems commonly
treated with traditional medicinal plants in Ethiopia. Echinops kebericho M. (Asteraceae) is the
one which is frequently used to treat pain and inflammation by traditional healers in Ethiopian
folk medicine. However, the plant has not been scientifically evaluated for its traditionally
claimed use. The present study aimed at the investigation of analgesic and anti-inflammatory
activities of 80% methanol root extract of Echinops kebericho M. in mice model.
Methods: Successive maceration was used as a method of extraction using solvents of
increasing polarity: methanol and water. After extraction of the roots with 80% hydro
methanol, the crude extract was evaluated for its peripheral and central analgesic activities
using acetic acid-induced writhing test and hot plate method, respectively, while its antiinflammatory
activity was evaluated using carrageenan- and formalin-induced paw edema.
The extract was evaluated at 100, 200 and 400 mg/kg doses. The positive control groups
were treated with ASA 150 mg/kg for writhing test, morphine 10 mg/kg for hot plat method,
indomethacin 25 mg/kg and diclofenac 10 mg/kg for paw edema tests and vehicle, distilled
water (10 mL/kg) treated mice were assigned as negative controls. All treatment administrations
were performed orally.
Results: E. kebericho extract at all test doses showed statistically significant antinociceptive
activity in both chemicals-induced peripheral and thermal-induced central pain in a dose dependent
manner (p < 0.01 and p < 0.001). The greater analgesic activity was observed by the
maximum dose of the extract (400 mg/kg) in both acetic acids-induced writhing test (57.84%)
and hot plate method (69.40%). The effect of the extract was also statistically significant (p <
0.01 and p < 0.001) in both carrageenan and formalin-induced paw edema in dose dependent
manner. Greater edema inhibition was observed by the highest dose (400 mg/kg) in both
observations with the respective percentage values of 70.00% and 79.87%, respectively.
Conclusion: In general, the data obtained from the present study elucidated that the extract
possessed a significant analgesic and anti-inflammatory activities and recommended for
further studies.
Keywords: analgesic activity, anti-inflammatory activity, Echinops kebericho
