DETERMINATION OF HEMATOLOGICAL REFERENCE INTERVALS HEALTHY ADULTS IN NORTHEAST ETHIOPIA

Abstract

ABSTRACT Background: Hematological reference interval is the range between two reference values that are used for interpretation of test results. It is affected by various physiological and environmental factors, thus locally derived hematological reference values are essential for accurate diagnosis and treatments of patients. Objective: The main objective of this study was to establish hematological reference intervals for healthy adults at Kemise, Northeast Ethiopia, 2019. Method: A cross-sectional study from apparently healthy blood donors from January to April 2019 was done in Kemise Blood Bank. The study participants were selected based on physical examinations and clinical history interviews using convenience sampling technique. About 3ml of blood for complete blood count was collected with tri-potassium ethylene diamine tetra-acetic acid containing test tube by antecubital vein puncture and analyzed using Sysmex XP-300 at Kemise General Hospital. The data was entered into Epi-Inf7 and then transferred to SPSS version 20 for analysis. Dixon and Reed 1/3 rule for outlier detection and Harris and Boyd for partition analysis were used. The 2.5th percentile (lower reference limit) and 97.5th percentiles (upper interval limits) were determined nonparametrically with 95% confidence interval. Result: A total of 329 study participants with median age of 23 years were recruited. The 2.5th- 97.5th percentiles reference interval, for parameters mainly red blood cell males: 3.9- 6×1012/L, females: 3.3-5.3×1012/L, hemoglobin males: 12-17.9 g/dl, females: 10.5-16.3 g/dl, and hematocrit males: 34.8-51.4%, females: 30.2-47%. The combined reference intervals for both sexes, were mean corpuscular volume: 77.1-95.5 fl. mean corpuscular hemoglobin: 25.6-34 pg, mean corpuscular hemoglobin concentration: 31.5-36.7 g/dl, red cell distribution width: 11.3-15.4%, white blood cell: 3.1-10.6×109/l, lymphocyte: 1-3.7×109/l, mixed: 0.3- 1.7×109/l, neutrophil: 1.4-7×109/l and platelet: 136-425×109/l. Conclusion: The established reference intervals in this study differ from other studies and in text books, and thus provided population specific hematological reference interval in Kemise. Reference intervals should also be established in the other regions of the country. Also,we recommened for health institutions which is found in Kemise to use the determined RI. Keywords: Hematological parameters, reference intervals, Kemise.