Abstract:
Abstract
Introduction: Diabetes mellitus is a metabolic disorder characterized by hyperglycemia with
disturbances of carbohydrate, fat, and protein metabolism, resulting from defects in insulin
secretion, insulin action, or both. Diabetes mellitus is one of the leading causes of death,
disability, illness, and economic loss worldwide. Managing diabetes mellitus with currently
available drugs is costly, and the chances of side effects are high, leading to further studies
for new and better medications from plant sources with the affordable and lower side effects.
Objective: To evaluate the antioxidant, antihyperglycemic and, antihyperlipidemic effects of
the 80% methanolic extract of Datura stramonium Linn (Solanaceae) leaves in
Streptozotocin-induced diabetic mice at University of Gondar, North West Ethiopia, 2022.
Material and Methods: Male Swiss albino mice were induced into diabetes using 150mg/kg
of STZ. Mice were allocated randomly into six groups, five mice per group. The body weight
and FBG measurements were done on 0, 7th, and 14th days of treatment. At the end of the
experiment, on the 14th day mice were anesthetized with diethyl ether. Then, blood was
drawn by cardiac puncture to assess TC, TG, LDL-C, and HDL-C. The antioxidant activity of
the extract was determined using a DPPH assay. The data were entered into Epi-Data version
4.6, exported to SPSS version 26.0, and analyzed using a one-way ANOVA followed by a
Tukey post hoc test. P<0.05 was considered statistically significant.
Result: The crude extract of the plant did not show any sign of toxicity up to 2 g/kg dose.
D.stramonium leaves extract showed significant blood-glucose-lowering activity as compared
to the diabetic negative control. The extract of D.stramonium reduced the fasting blood
glucose level by 19.71%, 30.27%, 40.95%, and 45.67%, respectively, for D. stramonium 100,
200, 400, and GLC 5 mg/kg on the 14th day of treatment. Diabetic mice treated with
D.stramonium (100, 200, and 400 mg/kg) for 14 days ??showed a significant decrease in
serum TC, LDL, and serum TG and a significant increase in body weight, and HDL level as
compared to diabetic negative control. Antioxidant activities of the leaves extract were
comparable to ascorbic acid with an IC50 of 172.79 ?g/ml.
Conclusion: This study showed that the 80% methanolic leaves extract of D. stramonium
possesses significant anti-hyperglycemic, antihyperlipidemic and antioxidant activities.
Keywords: Antihyperglycemic, Antihyperlipidemic, Antioxidant, Diabetes Mellitus, Datura
stramonium