Abstract:
Hot springs represent hot spots for identification of different industrial enzymes like lipases. There
has been an increased research interest in identifying and characterizing thermophilic
microorganisms from hot springs in the environment, contributing to the commercialization of
these industrial enzymes. Therefore, the aim of the present study was to isolate and characterize
thermophilic microorganisms of hot springs, which show high biotechnological and ecological
potential.Hot spring Water samples were collected from three thermal hot springs in south
Gondar zone Fogera woreda Dera-hamusit Kebele at Wanzaye local area. Water samples were
aseptically collected at the shallow part of the hot spring outlet by using 500 ml sterile thermal
glass containers and the samples were maintained inside the refrigerator until processing. The
temperature & pH of those hot spring samples were measured by using thermometer and pH
meter. Bacterial growth media was prepared aseptically. Lipase producing bacterial isolates
showed growth on the selective growth medium was further screened by using morphological,
biochemical test and their growth temperature difference. The results of the study indicate that
all the bacterial isolates from wanzaye hot spring had lipase activity and in addition to this they
had also additional enzymatic activities like cellulase, lectinase, protease and amylase.Sixteen
isolates with lipase activity were isolated from the Wanzaye hot spring. Among the sixteen
isolates, only m14b, s24a, m13b and s11a isolates were able to grow at high temperature (45°C).
Based on cultural, morphological and biochemical studies, the isolates were grouped under genus
Bacillus.
Key words:-Bacillus Spp, Hot spring, Lipase, Thermophilic
