PHYTOCHEMICAL INVESTIGATION, DETERMINATION OF ANTIMICROBIAL STUDY AND CHARACTERIZATION FROM BULB EXTRACT OF GLADIOLUS IRIDACEAE (YEJIBSHINIKURIT)

Abstract

Nearly 80% of the world’s population relies on traditional medicines for primary health care, most of which involve the use of plant extracts. In different parts of Ethiopia, people are using traditional herbal medicine for addressing their primary health care needs and to treat various ailments and physical damages. Most of the people of Ethiopia are highly dependent on plant-based traditional medical practices. Gladiolus Iridaceae is among the plant species used as traditional herbal medicines in Ethiopia for the treatment of uveitis, and hypertension and used as good antioxidant and activities. Phytochemical analysis and determination of antioxidant and antimicrobial activities of bulb extract of Gladiolus Iridaceae (YejibShinikurit) is the main target of this thesis. Bulb parts of Gladiolus Iridaceae will be extracted by using petroleum ether, chloroform, and methanol solvents successively and to estimate antioxidant capacities of bulb extracts Gladiolus Iridaceae, NMR and IR will be the selected methods. Bacteria of the extracts are determined by using the agar are method. Qualitative and quantitative phytochemicals of bulb extracts of Gladiolus Iridaceae are identified by using standard methods. Keywords: Traditional medicine, Gladiolus Iridaceae, Phytochemicals, Antimicrobial activity, Total phenolic, and flavonoid contents