PHYTOCHEMICAL, TOTAL PHENOL AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES DETERMINATION ON THE LEAVES EXTRACT OF Rhus vulgaris (KIMMO)

Abstract

Rhus vulgaris commonly known as sumac, a plant that is known to possess different therapeutic values including antioxidant and antibacterial activities. 800 g dried leaves of R.vulgaris were macerated sequentially with n-hexane, chloroform and methanol. The three extracts were separately subjected to preliminary phytochemical screening test and the result showed the presences of Alkaloids, Glycosides, Steroids, Anthraquinones, Carbohydrates, Phenols, Flavonoids, Tannins, Coumarins, and Proteins and the absence of Terpenoids, saponins and amino acids in the extracts. The extracts were also presented to Antibacterial activities by agar-well diffusion method against Staphylococcus aureus, Salmonella typhi, Escherichia coli and K.pneumoniae using Gentamicin as a positive control. The methanol extract showed better inhibition zones as compared to the standard. Total phenolic and flavonoid contents were done on the methanol extract by Folin-Ciocalteu reagent and Aluminum chloride; the results are 83.15 mg GAE/g and 24.246 mg CE/g respectively. The antioxidant activity of the methanol extract was also done by DPPH reagent and the result was 22.86 mg AAE/ g. From the methanol extract one major component was isolated by column chromatography and the compound was identified as 6-(13-(2-methylbutyl) tricosa-7, 14, 19-trienyl) cyclohexanol by spectroscopic techniques