Abstract:
Tuberculosis is an airborne infectious bacterial disease, which is caused by the bacillus
Mycobacterium tuberculosis and affects mainly the lungs & other sites of the body. It is
one of the biggest public health problems and the leading causes of death worldwide. An
increase in the frequency of Mycobacterium tuberculosis strains resistant to one or more
first line anti-TB drugs is a major concern in the increasing of TB burden and the
emergency of MDR TB worldwide. The general objective of this study was to evaluation
MTBDRplus assay for first line anti-tuberculosis drug resistance of mycobacterium
tuberculosis using MGIT 960 DST system on culture positive isolets at Gondar
University of Compressive Specialized HospitalTB Laboratory. So, the outcome of this
study was provide a current knowledge or information in Mycobacterium tuberculosis
and its drug resistance indicating genotypic and phenotypic on culture positive isolets for
national TB control program to control the spread and emergency of drug resistant TB in
Ethiopia. Laboratory based across sectional study design was conducted fromFebruary
to May in 2020. samples were collected from deep freeze and extracted by using genolize
& buffer solution, used the supernatant for PCR after that the DNA was amplified &
performed first line probe assay and phenotiypic DST. The data was analysed by using
SPSS version 20 statistical software. The results were presented through persentage,
tables and graph. A total of 53 clinical M. tuberculosis isolates were included in this
study. The majority of the study participants were males 35 (66%) & 7 ( 13. 2 % ) were
HIV positive.Two (3.8%) were MDR isolate that missed rpoBWT8 and WT katG gene and
developed rpoB MUT1 and katG MUT2. In conclusion, the present study showed the
genotypic characterization of anti-TB drug resistances as well as phenotypic test results
in MTBC strains from UOGCSH.both tests detected 2(3.8%) MDR-TB & 35(65.1%)
susceptible to INH and RIF
