Abstract:
In the present study, synthesis, characterization,
and the antibacterial activity of silver nanoparticles from
native isolate of Corynebacterium glutamicum has been
reported. Silver nanoparticles were synthesized by challenging
the dried biomass of C. glutamicum with aqueous
diamine silver ([Ag (NH3)2]?) containing 1 mM AgNO3.
Synthesized silver nanoparticles (AgNPs) were characterized
by ultraviolet–visible spectroscopy and energy-dispersive
X-ray (EDX) spectroscopy analysis. Morphological
study of silver nanoparticles was carried out using transmission
electron microscopy (TEM) and scanning electron
microscope (SEM). The spherical morphology of silver
nanoparticles was confirmed from SEM image. The TEM
image showed the average particle size of silver nanoparticles
was about 15 nm. Silver nanoparticles synthesized
from C. glutamicum were found to have enhanced antimicrobial
activity against selected pathogenic strains. Silver
nanoparticles from pure strains of Corynebacterium
species was done by many investigators, but as per the
present literature, this is the first report on the production of
silver nanoparticles using a native strain of
Corynebacterium.