Impact ofERCC2gene polymorphism on HIV-1 disease progression to AIDS among North Indian HIV patients

Abstract

HIV/AIDS remains to be one of the killing diseases of mankind. Host genetic response is one of the factor which determine susceptibility to HIV and disease progression to AIDS. The aim of the present study was to evaluate the impact ofERCC2 Lyc 751 Gln(excision repair cross complementing rodent repair deficiency, complementation group 2) polymorphism on HIV-1 susceptibility and disease progression to AIDS, as this gene has been reported to intervene in degrading retroviral cDNA before it integrates with the host DNA. This case control study included 300 HIV seropositive cases and an equal number of HIV seronegative controls. DNA was isolated from the blood samples of study subjects and genotyping ofERCC2 was conducted by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. The Gln/Glngenotype showed a significant variation between cases and controls (P=0.047, OR 1.71, 95% CI 1.00–2.93), indicating a possible role of susceptibility in reference to controls and disease progression when compared within cases