ASSOCIATION OF TETA AND TETB GENES AND DRUG RESISTANCE PATTERN OF K.PNEMONIAE ISOLATED FROM PATIENTS VISITING GONDAR UNIVERSITY TEACHING HOSPITAL

Abstract

Klebsiella pneumoniae found as a common flora in intestinal tract, mouth and skin of humans do not cause disease. However, when the bacterium founds other parts of the body, it is associated with many different types of human disease. K. pneumoniae is one of the pathogen that contains a variety of resistance genes. The objective of this study was to detect tetA and tetB resistance genes in K. pneumoniae. Experimental study was conducted on a totality of 50 K. pneumoniae isolates from different specimens of patients at Gondar University hospital from November, 2014 to April, 2016. The Klebsiella pneumoniae genomic DNA was isolated by phenol chloroform method. PCR was performed to detect tetA and tetB genes by specific pair of primers. K. pneumoniae showed resistance against Amoxicillin 50(100%), followed by Streptomycin 23 (60%). From total, forty (80%) of K. pneumonia isolates were multidrug resistance which showed a resistance to two or more antibiotics. The occurrence of the genes tetA and tetB were 2 (4%) and 1(2%) for tetA tetB genes, respectively. The presence of multidrug resistance K. pneumoniae strains that harbor resistance genes (tetA and tetB), might be serving as indicators for the role of these genes in antibiotic resistance. Key words: Klebsiella pneumoniae, resistance, tetA, tetB, tetracycline